ESSENTIAL BIOMARKERS

1. HORMONE PANELS

MEN

Total Testosterone

Measures total circulating testosterone.
Why it matters: Determines overall androgen status; low levels affect libido, muscle, fat loss, energy, mood.

Free Testosterone

Unbound, active testosterone.
Why it matters: More accurate predictor of symptoms than total T.

Bioavailable Testosterone

Portion available to tissues.
Why it matters: Helps determine if high SHBG is blocking testosterone utilization.

SHBG (Sex Hormone Binding Globulin)

Protein that binds hormones.
Why it matters: High SHBG → low usable testosterone; Low SHBG → metabolic issues.

Estradiol (E2 – Sensitive Assay)

Estrogen level in men.
Why it matters: Too high = mood swings, gyno; too low = joint pain, low libido.

DHEA-S

Precursor to sex hormones.
Why it matters: Declines with age; supports energy, mood, and androgen balance.

LH & FSH

Pituitary hormones stimulating testicles.
Why it matters: Distinguishes primary vs secondary hypogonadism.

Prolactin

Pituitary hormone.
Why it matters: High levels can suppress testosterone and libido.

Pregnenolone

Parent hormone for all steroid hormones.
Why it matters: Linked to memory, mood, stress response.

DHT (Dihydrotestosterone)

Potent androgen.
Why it matters: Controls libido, strength; too high may contribute to hair loss.

PSA (Prostate-Specific Antigen)

Prostate biomarker.
Why it matters: Required for safe TRT monitoring.

WOMEN

Estradiol (E2)

Primary female hormone.
Why it matters: Controls mood, metabolism, libido, menstrual cycle, bone health.

Progesterone

Key calming hormone.
Why it matters: Low levels → anxiety, PMS, infertility, sleep issues.

Total Testosterone

Overall androgen level.
Why it matters: Supports libido, strength, and energy.

Free Testosterone

Active testosterone.
Why it matters: More accurate for symptoms like low libido, fatigue, weight gain.

DHEA-S

Hormone precursor.
Why it matters: Anti-aging, improves mood, cognition, and adrenal function.

FSH / LH

Ovulation and ovarian function markers.
Why it matters: Critical for fertility and diagnosing hormonal imbalance.

Prolactin

Regulates menstrual cycle.
Why it matters: High levels → amenorrhea, infertility.

SHBG

Hormone binding protein.
Why it matters: High in women with low energy or under-eating; affects testosterone.

Pregnenolone

Neurosteroid precursor.
Why it matters: Supports cognition and stress resilience.

AMH (Anti-Müllerian Hormone)

Ovarian reserve marker.
Why it matters: Important for fertility, menopause prediction.

2. THYROID FUNCTION (MEN & WOMEN)

TSH (Thyroid Stimulating Hormone)

Master thyroid regulator.
Why it matters: Elevated levels suggest hypothyroidism; important for metabolism.

Free T3

Active thyroid hormone.
Why it matters: Low T3 = fatigue, weight gain, hair loss, slow metabolism.

Free T4

Thyroid hormone precursor.
Why it matters: Helps determine thyroid gland output.

Reverse T3 (rT3)

Inactive thyroid hormone.
Why it matters: High rT3 blocks T3 → common in chronic stress, dieting, illness.

TPO Antibodies

Autoimmune thyroid marker.
Why it matters: Detects Hashimoto’s disease before symptoms appear.

Thyroglobulin Antibodies (TgAb)

Another autoimmune thyroid marker.
Why it matters: Confirms autoimmune thyroid dysfunction.

3. METABOLIC & INSULIN SENSITIVITY MARKERS

Fasting Insulin

Shows insulin sensitivity.
Why it matters: Best early detector of prediabetes + weight-loss resistance.

Fasting Glucose

Blood sugar at baseline.
Why it matters: Elevated = metabolic dysfunction.

HbA1c

3-month blood sugar average.
Why it matters: Predicts diabetes risk.

C-Peptide

Insulin production indicator.
Why it matters: Helps identify insulin resistance vs insulin deficiency.

HOMA-IR

Insulin resistance index.
Why it matters: Strong predictor of metabolic disease.

ApoB (Apolipoprotein B)

Primary LDL particle marker.
Why it matters: BEST cardiovascular risk marker; better than LDL.

Lipoprotein(a)

Genetic cholesterol subtype.
Why it matters: Strong predictor of early heart disease.

4. INFLAMMATION & LONGEVITY MARKERS

hs-CRP (High Sensitivity C-Reactive Protein)

Systemic inflammation.
Why it matters: Higher levels → aging, heart disease, metabolic issues.

ESR (Sed Rate)

General inflammation marker.
Why it matters: Elevated in infections, autoimmune disorders.

Fibrinogen

Clotting protein.
Why it matters: Elevated levels raise stroke and heart attack risk.

Homocysteine

Amino acid affecting methylation.
Why it matters: High levels → cardiovascular risk + B vitamin deficiency.

Uric Acid

Metabolic waste product.
Why it matters: High levels = cardiovascular and metabolic risk.

Cortisol (AM/PM)

Stress hormone.
Why it matters: Chronic high cortisol → weight gain, burnout, aging acceleration.

5. LIVER, KIDNEY & DETOX MARKERS

Liver

AST / ALT

Liver cell enzymes.
Why it matters: Elevated from alcohol, fatty liver, medications, or toxicity.

Alkaline Phosphatase

Bile flow marker.
Why it matters: Indicates liver, bone, or gallbladder issues.

GGT

Oxidative stress toxin marker.
Why it matters: Top marker for alcohol damage and toxin load.

Bilirubin

Red blood cell breakdown.
Why it matters: Indicates liver and gallbladder health.

Kidney

Creatinine

Waste product filtered by kidneys.
Why it matters: Elevated = impaired kidney filtration.

BUN

Nitrogen waste product.
Why it matters: Helps assess hydration + kidney function.

eGFR

Overall kidney function.
Why it matters: Required to assess kidney disease risk.

6. LIPID & CARDIOVASCULAR MARKERS

Total Cholesterol

General lipid status.
Why it matters: Foundation of cardiovascular risk.

HDL

“Good” cholesterol.
Why it matters: Higher = protective.

LDL (Direct)

“Bad” cholesterol.
Why it matters: Helps risk-stratify; must be paired with ApoB.

Triglycerides

Blood fats.
Why it matters: High levels = metabolic dysfunction + insulin resistance.

VLDL

Very low density lipoproteins.
Why it matters: Linked to high triglycerides / liver issues.

LDL Particle Count (LDL-P)

Advanced lipoprotein marker.
Why it matters: More predictive of plaque formation.

7. NUTRIENT, ELECTROLYTE & MITOCHONDRIAL MARKERS

Vitamin D

Critical hormone-like vitamin.
Why it matters: Low = weak immunity, weight gain, depression.

Vitamin B12

Energy metabolism + nerves.
Why it matters: Deficiency → fatigue, neuropathy.

Folate (RBC Folate)

Methylation + DNA repair.
Why it matters: Essential for longevity + pregnancy.

Magnesium RBC

Intracellular magnesium.
Why it matters: Regulates energy, muscles, sleep, stress.

Iron Panel (Ferritin, Iron, TIBC, Transferrin Sat.)

Oxygen delivery.
Why it matters: Low iron → fatigue; high iron → inflammation, aging.

Zinc / Copper Ratio

Mineral balance.
Why it matters: Key for hormones, immunity, skin health.

CoQ10

Mitochondrial enzyme.
Why it matters: Essential for energy production; depleted by statins.

Omega-3 Index

EPA + DHA percentage.
Why it matters: Low levels = high inflammation + cardiovascular risk.

8. LONGEVITY & CELLULAR HEALTH

IGF-1

Growth hormone activity.
Why it matters: Low levels = aging; too high = cancer risk.

IGFBP-3

Binding protein.
Why it matters: Helps interpret GH status accurately.

Telomere Length

Genetic aging marker.
Why it matters: Short telomeres = accelerated aging.

NAD+/NADH Ratio

Cellular energy/repair marker.
Why it matters: Declines with age; correlated with mitochondrial health.

8-OHdG (Oxidative Stress)

DNA oxidation marker.
Why it matters: High levels = high oxidative aging.

9. REPRODUCTIVE MARKERS

Men

Semen Analysis

Fertility and testicular function.

FSH / LH

Determines sperm production health.

Prolactin

High levels suppress testosterone and fertility.

Women

AMH

Ovarian reserve.

FSH / LH

Cycle regularity + menopause transition.

Day-21 Progesterone

Ovulation confirmation.

10. SPECIALTY BIOHACKING ADD-ONS

Myokines (Irisin, Myostatin)

Muscle signaling proteins.
Why it matters: Myostatin inhibits muscle growth; Irisin boosts fat burning.

Adiponectin / Leptin

Metabolic hormones.
Why it matters: Regulate weight, appetite, insulin sensitivity.

Gut Microbiome Panel

Gut ecosystem.
Why it matters: Impacts immunity, mood, weight, inflammation.

Heavy Metals

Toxins like mercury, lead.
Why it matters: Causes fatigue, hormonal imbalance, aging.

Mold/Mycotoxin Panel

Environmental toxins.
Why it matters: A cause of fatigue, hormonal issues, inflammation.

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